Journal: Reproductive Biology and Endocrinology : RB&E
Article Title: Growth differentiation factor-11 downregulates steroidogenic acute regulatory protein expression through ALK5-mediated SMAD3 signaling pathway in human granulosa-lutein cells
doi: 10.1186/s12958-022-00912-7
Figure Lengend Snippet: GDF-11 expression levels are not varied in hGL cells and follicular fluid between normal and PCOS patients. A , and B , Follicular fluid samples were collected from 36 non-PCOS and 36 PCOS patients of similar age. The body mass index (BMI), antral follicle count (AFC), and basal levels of hormones in serum including follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P4), and testosterone (T) were measured. C , GDF-11 protein levels in follicular fluid samples were measured by ELISA. D , GDF-11 mRNA levels in hGL cells obtained from 12 non-PCOS and 12 PCOS patients were measured by RT-qPCR. The clinical results are expressed as the mean ± SD
Article Snippet: GDF-11 protein levels in follicular fluid were measured using an ELISA Kit (#E-EL-H1908, Elabscience) as per the manufacturer’s instructions.
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR